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Afterwards, the cells were blocked, permeabilized and incubated with primary mouse monoclonal antibody to γ H2AX (Ser-139) at 1 : 1000 (Upstate Biotechnology, Upstate, NY, USA) and diluted 1 : 500 for 1 h at room temperature, followed by incubation with anti-mouse FITC secondary antibody (Sigma-Aldrich) that was diluted 1 : 200 for 1 h at room temperature

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