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Follow-up Repeat broad markers after 6-12 weeks if the protocol continues, symptoms change, or there are concerns about inflammation, infection, bruising, swelling, or recovery quality

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In a model of depression induced by chronic unpredictable stress, the expression of tryptophan hydroxylase 1 (TPH1)—the enzyme crucial for 5-HT synthesis—was markedly downregulated in intestinal EC cells, resulting in a decreased colonic 5-HT concentration