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The primary forms of one-carbon units include: Methyl (-CH₃) Groups Methyl groups are the most prevalent form of one-carbon units and play a pivotal role in methylation reactions, where they are transferred to various substrates, including DNA, RNA, and proteins

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Statistical analysis Comparisons between experimental groups were conducted using repeated measure analysis of variance (ANOVA) followed by the Student–Newman–Keuls post-hoc test