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Under the same experimental conditions, the RPE-1 cell line showed a significant increase in only the HO-1 gene expression, suggesting that these cells might elicit a different cytoprotective mechanism in response to CPZ-induced oxidative stress and that might be more capable of recovering the damage

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From Day 10, neuronal maturation media, consisting of DMEM-F12/Glutamax, N2 supplement, B27 supplement, BDNF (20 ng/ml), Lam (1 μg/ml), GDNF (20 ng/ml, Peprotech 450-10), dibutyryl-cAMP (500 μg/ml, Selleckchem S7858), ascorbic acid (200 nM, Sigma A4544), AraC (2 μM), and P/S (1X) was used with half media changes every 2–3 days