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10.1186/s12958-020-00631-x 56 YangJ.LiY.LiS.ZhangY.FengR.HuangR.et al (2022)

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Prior to experimentation, the enzyme was thawed at room temperature and small amounts of precipitated enzyme were removed by centrifugation at 10,000 g for 10 min, and 30 kDa centrifugal filters were used to buffer exchange the supernatant enzyme into the desired reaction buffer (typically 50 mM KPi, pH 7.4)