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Studies have shown that the plasma markers of leucocyte activation and leucocyte-endothelial interaction (L-selectin, intercellular adhesion molecule (ICAM)-1, endothelial leukocyte-adhesion molecule (ELAM)-1, vascular-cell adhesion molecule (VCAM)-1 are increased after subjecting patients with varicose veins to 30 minutes of standing to induce venous hypertension (Figure 3) [94, 95]