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Torsional energy calculations were performed using an online torsional energy calculator 1 based on input dihedrals, which uses a combined quantum chemical ( χ 2 , χ 3 , χ ′ 2 ) and empirical calculation ( χ 1 , χ 1 ′) described in detail elsewhere (Haworth et al., 2

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Isothermal titration calorimetry Experiments were carried out on a MicroCal auto-ITC200 instrument at 25 °C while stirring at 750 rpm in the ITC buffer of pH 6.5, consisting of 100 mM sodium phosphate, 2 mM EDTA and 2 mM β-mercaptoethanol