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This measurement could not be performed with [1- 13 C]-DHA due to the rapid hydrolysis of the sample, with a half-life of approximately 10 min at neutral pH.(23) The T 1 of the hyperpolarized 13 C label was determined in a separate experiment at pH 7.0 using 180 spectra acquired with an 8° flip angle pulse (nt = 1, 16 kHz spectral window, T R = 1 s, total acquisition time of 3 min) and fitting the decay of the integrated peak intensity to eq 1: where S 0 is the initial integrated peak intensity, T R is the repetition time in seconds, T 1 is the spin–lattice relaxation time in seconds, α is the flip angle, and n is the number of preceding RF pulses
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Dopamine and citicoline-co-loaded solid lipid nanoparticles as multifunctional nanomedicines for Parkinson’s disease treatment by intranasal administration
