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Briefly, 3 µg of each protein sample was mixed with 15 µL of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 µL of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 µL of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark