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The LBD-TMD assembles were resolved by subtracting ATD signals from the particles, followed by ab initio reconstruction, and several rounds of heterogeneous refinements to obtain a uniform particle subset, and then a final round of non-uniform refinements to result in a resolution of 4.39 Å (intact LBD, C2 symmetry), 4.22 Å (partially ruptured state 1), and 4.27 Å (partially ruptured state 2)

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