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Further, when we examined subcellular localization of the lipid ROS probe by confocal imaging (the probe changes fluorescence from red to green upon oxidation), we found in both HT1080 cells and mouse embryonic fibroblasts (MEFs) treated with erastin, the oxidized probe first appeared in a distribution that significantly colocalized with mitochondria, and then colocalized with the plasma membrane at later time points (Figure 1E and Figure S1)

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The cellularity of the TM declines linearly with a loss of 0.58% of cells per year and a 47% decline in absolute cell number over an 81-year study period [41]