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Early recipes for pita bread (first varieties used emmer/farro or sometimes barley flour) also recommend that the dough be left to rest for hours to ferment slightly before baking

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Single bacterial colonies were isolated, and their 16S rRNA genes were amplified by PCR using universal bacterial primers 27 F (5’ AGAGTTTGATCCTGGCTCAG 3’) and 1492 R (5’-GGTTACCTTGTTACGACTT-3’)