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Materials and methods Reagents and antibodies Reagents used in this article include: Puromycin dihydrochloride (#B7587, ApexBio), Polybrene (#H8761, Solarbio), MG132 (#HY-13259, Med Chem Express), Cycloheximide (#HY-12320, Med Chem Express), Triton X-100 (#T8200, Solarbio), Proteinase K Solution (#P1120, Solarbio), Sucrose (#S8271, Solarbio), Bovine Serum Albumin (#ST023, Beyotime), Anti-HA beads (#C0323, BioMag), Protein A + G Agarose (#P2055, Beyotime), Anti-FLAG® M2 Affinity Gel (#A2220, Millipore), FLAG Peptide (#T510060, Sangon Biotech), Anti-Diglycine Lysine Antibody Conjugated Agarose Beads (#PTM-1104, PTM BIO), Pierce™ GST Protein Interaction Pull-Down Kit (#21516, Thermo Fisher Scientific)
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Ipamorelin is the preferred secretagogue for this combination due to its selectivity
