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GIRK channels possess two operational gates–an inner helix gate (also known as helix bundle crossing gate, formed by four F192 side chains in the inner helices of GIRK2) and a G-loop gate (formed by hydrophobic residues on the βH-βI loop of the CTD) (Pegan et al., 2005)–both of which are controlled by a combination of stimuli originating from the cytoplasmic and membrane environments (Whorton and MacKinnon, 2011)