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Functional characterization of hTrxR1 variants In order to study the impact of the guiding bar residues (Trp407′ hTrxR1 , Asn418′ hTrxR1 , and Asn419′ hTrxR1 ) of hTrxR1 upon catalysis, we constructed two different mutants of hTrxR1: hTrxR1 N419A,U498C , and hTrxR1 W407A,N418/9A,U498C (Table 2)