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A: Pull-down assays can detect weak interactions (μM affinity range) by increasing bait concentration and reducing wash stringency

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Fluoxetine hydrochloride (Amoun Pharmaceutical Co., Cairo, A.R.E.), sertraline hydrochloride (Pfizer Egypt, Cairo, A.R.E.) and imipramine hydrochloride (Novartis Pharma, Cairo, A.R.E.) were used and dissolved in isotonic (0.9% NaCl) saline solution immediately before use