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The application of single cell methods in human studies will also be critical, in particular to define the nuanced effects of fasting on the immune system, as it is clear from studies in model organisms that fasting not only affects the cellular phenotypes of immune cells in distinct ways (eg, promoting M1 to M2 phenotypic switching of macrophages), but may also modulate cell trafficking and in turn the cellular composition of both circulating and tissue immune cell reservoirs (226, 260, 261)

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Proc Natl Acad Sci 96 (9): 5292–5297